'Chromatography Lab Repot\r'

' inclination and carry out a scientific investigation on any sensation federal agent that affects memory on root word chromatography Design and carry out a scientific investigation on any wiz factor that affects retention on report chromatography CHEMISTRY LAB embrace CHEMISTRY LAB REPORT Name: Sri Danielle yr: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay invest My aim for this experiment is to find out how dissimilar types of dis declaration result affect the retention factor in paper chromatography. HypothesisI think that there will be a high RF tax in paper chromatography, if we use urine solvent because a solvent that has a strong interaction with a chemical will easily all over come attraction from the chemical for the absorbent tier and move that chemical in a given(p) period of time. Materials Food Coloring (solute) Ethanol, Acetone, Tap Water, Distilled Water(solvents) assume Ruler Scissors Filter Paper Petri mete out Variables Ind ependent: Type of chromatography solvent Dependent: guardianship in paper chromatography Controlled: Type of Chromatography paperSize of spot ponderousness of Chromatography Substance tested for paper chromatography Concentration of the solute Temperature Variables manipulation Independent: Apply diverse solvents Dependent: The RF prise of the given solvent Controlled: hold open the solute same. Keep the size of the solution the same (one drop) Keep the temperature of the means the same. Diagram Method mensuration 1 †acquire Materials and pour solvents into the petri dish. bar 2- Fold the provided chromatography paper into half(prenominal) (make sure it can stand) measuring 3- measure 1. cm from one end of the paper then draw a berth across. This will be apply as a starting point. Step 4- recall for 4 chromatography papers for each solvent. Step 5- go forth a drop of the solution on the pencil line for all 4 chromatography papers Step 6- Mark the chromatography paper to identify it’s solvent Step 7- Fill the 4 different petri dishes with one of each of the solvents Step 8- Make sure the solvents do not reach the pencil line Step 9- Observe as the chromatogram developsStep 10- Make sure in the beginning you take out the chromatography paper, it is entirely wet by the solvent Step 11- Draw a line along the line up to which the solvent has travelled on each chromatography Step 12- Measure the outperform between the two pencil lines on the chromatography paper and record the measurement on the top of the paper. Do this for each paper. Step 13- Measure the RF revalue by dividing â€Å"distance travelled by destiny” with â€Å"distance travelled by solvent Step 14- Record the measurement then tabulate the info. Results center| withdrawnness travelled by component| Distance travelled by solvent| RF pass judgment| Tap Water| 5. | 6. 4| 0. 90| Distilled water| 7. 0| 7. 2| 0. 97| Acetone| 4. 7| 5. 5| 0. 85| Ethanol| 3. 1| 6. 1| 0. 50| Graph Reflection In this experiment, I put two drops on each paper; I also used Tap water and Distilled water to get a more tried result for the solvent. This helped me get me more undefiled result. It was delicate to get the RF value as I did not get he exact distances (distance travelled by component and solvent) as I got upturned on how to take the reading properly. If I do this experiment again, I will surely command a teacher on how to do so and to get a better and more accurate result.Conclusion According to the results of the experiment, my hypothesis was proved to be correct. The data shows that the water solvent results as the highest RF value among all the other solvents. It also shows that the solvent results in a great affect retention of chromatography paper. The different types of solvents affect the retention in paper chromatography. In all the solvents I used, water has the greatest effect in retention, as its RF value creation the highest among all. Precaution 1. Careful when handling fragile materials 2. tangle with’t taste or smell solvents 3. make do chemicals with care\r\n'